opa1 nbp2 59770ss antibody Search Results


opa1 nbp2 59770ss antibody  (Novus Biologicals)
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Novus Biologicals opa1 nbp2 59770ss antibody
Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and <t>OPA1</t> protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.
Opa1 Nbp2 59770ss Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opa1+nbp2+59770ss+antibody/OPA1+Antibody+(1284B)+-+BSA+Free/pmc08275580-199-1-7
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opa1 nbp2 59770ss antibody - by Bioz Stars, 2026-10
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Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.

Journal: Scientific Reports

Article Title: Mitofusion is required for MOTS‐c induced GLUT4 translocation

doi: 10.1038/s41598-021-93735-2

Figure Lengend Snippet: Treatment of U-2 OS cells with MOTS-c initiated mitochondrial fusion. Treatment with MOTS-c increased MfN2 and OPA1 protein expression in ( A , B ) whole cells and ( C , D ) mitochondria. U-2 OS cells grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) plus 1% pen-strep were treated with vehicle (Nuclease-Free Water) or MOTS-c (25, 50 or 100 μM) for 48 h and western blot was performed to quantify the protein expression level of selected biomarkers. Mitochondria were extracted from cells treated with 100 μM MOTS-c only. GAPDH was used as the internal control for whole cell analysis while TOMM20 was used as the mitochondrial control. All full-length blots are presented in Supplementary Figure . Data are means ± SEM of three to four experiments. Statistical analysis was conducted using ordinary one-way ANOVA followed by Dunnett's multiple comparisons test vs vehicle; results are presented as *p < 0.05, and **p < 0.01 vs. vehicle.

Article Snippet: The OPA1 (NBP2-59770SS) antibody was obtained from Novus Biologicals (Toronto, Canada).

Techniques: Expressing, Modification, Western Blot, Control, Cell Analysis